Please join us for this week’s Biostatistics & Medical Informatics seminar on Friday!
Time & Place: Friday, October 30th, 2020 at 12:00 noon.
Title: Computational 3D Fluorescence Microscopy
Presenter: Dr. Laura Waller, Department of Electrical Engineering/Computer Science, UC-Berkeley
Abstract: We describe a compact and inexpensive computational microscope that encodes 3D information into a single 2D sensor measurement, then exploits sparsity to reconstruct the volume with good resolution across a large volume. Our system uses simple hardware and scalable software for easy reproducibility and adoption. The inverse algorithm is based on large-scale nonlinear optimization with self-calibration of aberrations and we discuss computational optical design approaches for optimizing the system’s performance. We demonstrate applications in whole organism bioimaging and neural activity tracking in vivo.